human liposarcoma cell lines sw872  (ATCC)

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: IL-4-Induced M2 macrophage polarization promotes the progression of LPS. RAW264.7 cells were divided into two groups: control group, IL-4 group. (A) Flow cytometry was used to detect the expression level of CD206 on RAW264.7 cells in each group. (B) ELISA was performed to measure the secretion level of IL-10 in RAW264.7 cells from each group. (C) Western Blot was employed to determine the protein expression level of Arg-1 in RAW264.7 cells of each group. A Transwell co-culture system was utilized, which included two groups: control co-culture group (LPS cells co-cultured with untreated RAW264.7 cells). IL-4 co-culture group (LPS cells co-cultured with RAW264.7 cells pre-treated with IL-4). (D) EdU incorporation assay was used to detect the proliferative activity of SW872 cells and 94T778 cells in each group. (E-F) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 cells and 94T778 cells in each group. Data are presented as the mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Control, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Western Blot, Co-Culture Assay, Cell Culture, Activity Assay, Migration

Journal: Adipocyte

Article Title: mTORC2 regulates lipid metabolism-driven TAMs via the PPAR-γ/CD36 pathway to promote liposarcoma progression

doi: 10.1080/21623945.2026.2665903

Figure Lengend Snippet: mTORC2 regulates TAM via PPAR-γ/CD36 pathway to promote LPS progression. LPS cells (SW872 and 94T778) were co-cultured with RAW264.7 macrophages, and the groups were divided as follows: control co-culture group, IL-4 co-culture group, IL-4+JR-AB2-011 co-culture group, IL-4+JR-AB2-011+LPA co-culture group. (A) EdU assay was used to detect the proliferation of SW872 and 94T778 cells in each co-culture group. (B-C) Transwell migration and invasion assays were performed to evaluate the migration and invasion abilities of SW872 and 94T778 cells in each co-culture group. Data are presented as the mean±SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Experiments were repeated independently at least three times.

Article Snippet: Human liposarcoma cell lines SW872 and 94T778, as well as the murine macrophage cell line RAW264.7, were purchased from the American Type Culture Collection (ATCC, USA).

Techniques: Cell Culture, Control, Co-Culture Assay, EdU Assay, Migration

Journal: Frontiers in Immunology

Article Title: Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma

doi: 10.3389/fimmu.2024.1372692

Figure Lengend Snippet: Validation of the function of TNFSF14 by in vitro experiments. (A) TNFSF14 overexpressed was confirmed by western blot assays in SW872 and HT1080 cells. (B, C) CCK-8 assays showed reduced cell proliferation in TNFSF14 overexpressed cells. (D–G) Cell migration and invasion of sarcoma cells were determined by Transwell assays. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, ns, no significance.

Article Snippet: In this experiment, the human liposarcoma cell line SW872 (CL-0685B) and the human fibrosarcoma cell line HT1080 (CL-0117) were purchased from Procell Biotech (Wuhan, China), and passed STR identification and mycoplasma contamination testing.

Techniques: Biomarker Discovery, In Vitro, Western Blot, CCK-8 Assay, Migration

Journal: Folia histochemica et cytobiologica

Article Title: Effects of bone marrow mesenchymal stem cell-conditioned medium on the proliferation and migration of liposarcoma cells.

doi: 10.5603/fhc.98379

Figure Lengend Snippet: Figure 3. Knockdown of YAP1 in BMSCs induces cell apoptosis in liposarcoma cell lines. A, B. Representative results of flow cytometric analysis of cell apoptosis in 93T449 and SW872 cells treated with shYAP1-BMSC-CM. C. Analysis of the rate of apo- ptosis in 93T449 and SW872 cells treated with shYAP1-BMSC-CM. The experiment was repeated twice. **P < 0.01 vs. control.

Article Snippet: Human liposarcoma cell lines 93T449 and SW872 (ATCC, Manassas, VA, USA) were maintained in DMEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), penicillin (100 mg/mL), and streptomycin (100 mg/mL; Invitrogen, Grand Island, NY, USA).

Techniques: Knockdown, Control

Journal: Folia histochemica et cytobiologica

Article Title: Effects of bone marrow mesenchymal stem cell-conditioned medium on the proliferation and migration of liposarcoma cells.

doi: 10.5603/fhc.98379

Figure Lengend Snippet: Figure 4. Knockdown of YAP1 in BMSCs impairs migration in liposarcoma cell lines. A, C. Representative migration images of 93T449 and SW872 cells treated at different time points (0, 8, 24, and 48 h) with shYAP1-BMSC-CM. Original magnification: 100×, Scale bar = 100 μm. B, D. Relative wound closure of cultured 93T449 and SW872 cells treated at different time points (0, 8, 24, and 48 h) with shYAP1-BMSC-CM. The experiment was repeated three times. **P < 0.01 vs. control.

Article Snippet: Human liposarcoma cell lines 93T449 and SW872 (ATCC, Manassas, VA, USA) were maintained in DMEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), penicillin (100 mg/mL), and streptomycin (100 mg/mL; Invitrogen, Grand Island, NY, USA).

Techniques: Knockdown, Migration, Cell Culture, Control

Journal: Folia histochemica et cytobiologica

Article Title: Effects of bone marrow mesenchymal stem cell-conditioned medium on the proliferation and migration of liposarcoma cells.

doi: 10.5603/fhc.98379

Figure Lengend Snippet: Figure 5. Knockdown of YAP1 in BMSCs results in the altered expression of related protein in liposarcoma cell lines. A. Represen- tative western blot analysis after treatment of 93T449 and SW872 cells with shYAP1-BMSC-CM. B, C. Downregulation of Bcl-2 and MMP-2 expression after treatment with shYAP1-BMSC-CM in 93T449 and SW872 cell lines as determined by semi-quantitative analysis. The experiment was repeated twice. **P < 0.01 vs. control.

Article Snippet: Human liposarcoma cell lines 93T449 and SW872 (ATCC, Manassas, VA, USA) were maintained in DMEM (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO, USA), penicillin (100 mg/mL), and streptomycin (100 mg/mL; Invitrogen, Grand Island, NY, USA).

Techniques: Knockdown, Expressing, Western Blot, Control